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Pursuant to Section 20 C.F.R. 656.10, you are hereby notified that an application for Alien Employment Certification will be filed by Takeda Development Center Americas, Inc. for the following job opportunity:
JOB LOCATION: Cambridge, MA
POSITION: Associate Scientist
POSITION DESCRIPTION : Takeda Development Center Americas, Inc. is seeking an Associate Scientist with the following duties: Spearhead the development and deployment of high-throughput platforms to accelerate early discovery of engineered γδT cell therapies. Establish scalable workflows using automated liquid handling and high-content imaging platforms to streamline construct screening across primary donor sources. Implement robotic protocols for cell isolation, transduction, and expansion in 96 & 384 well formats, to enable parallel testing of CAR variants with consistent throughput and reproducibility. Execute high-throughput screening of antibody discovery campaigns to identify lead candidates for CAR binder design and optimization. Perform high-throughput array-based pooled screening to evaluate novel CAR signaling domain constructs for functional activity. Design, execute, and optimize high throughput screening in vitro assays for lead hit identification —including cytokine profiling, 2D/3D cytotoxicity, and phenotypic flow cytometry. Collaborate with automation engineers and data scientists to troubleshoot workflow issues, validate assay performance, and drive seamless integration of experimental outputs into global systems. Develop standardized SOPs, defined QC metrics, and contribute to global harmonization efforts to support assay transferability and alignment across biologics and cell therapy pipelines. Mentor junior scientists, manage laboratory resources, and coordinate cross-functional project timelines within a matrixed team structure. Present key findings to cross-functional stakeholders to support early go/no-go decisions and alignment with pipeline strategy and IND-enabling milestones.
REQUIREMENTS: Master’s of Biology, Biotechnology degree or related field plus 3 years of related experience OR Bachelor’s of Biology, Biotechnology degree or related field plus 5 years of related experience. Prior experience must include: design and execute experiments to assess anti-tumor functionality of allogeneic innate CAR cell therapies (e.g. Cord blood derived CAR-NK, CAR-gdT), including analysis of cytokine, cytotoxicity assays, and repeat antigen stimulation; perform cell isolation, activation, transduction, expansion, and cryopreservation of PBMC-derived CARabT cells for functional characterization; exposure to automated or semi-automated liquid handling for in vitro functional assay setup; perform cell isolation, activation, transduction, expansion, and cryopreservation of cord-blood derived NK cells, ensuring high viability and functionality; perform gamma retroviral vector production and retroviral transduction of innate immune cells (e.g. NK or gdT); conduct multiparameter flow cytometry (8+ color) to characterize immune phenotypes; cell culture, immunofluorescence imaging & plasmid cloning.
Full time. $99,500.00 - $170,600.00 per year. Competitive compensation and benefits.
Qualified applicants can apply at . Please reference job #R0163160. EOE
Any individual may provide documentary evidence bearing on this application, with information on available workers and information on wages and working conditions, to the Certifying Officer, U.S. Department of Labor, Employment and Training Administration, Office of Foreign Labor Certification, 200 Constitution Avenue, NW, Room N-5311, Washington, DC 20210.
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